Mr. Harshal Zope

Cambridge

Qualification - PhD

Mobirise

About -

Principal Scientist at Genocea

Post Doctorate Pharmaceutical Sciences Harvard Medical School

PhD Pharmaceutical Chemistry, Leiden University

MSc, M.Tech Biotechnology, University of Pune

Narkhede, Highschool, Bhusawal 1991 – 2001

About

  • Developed drug delivery systems such as liposomes, polymer nanoparticles, and micelles for the delivery of small molecules, peptides and its conjugates, antibody conjugates, adjuvants, and oligonucleotides such as siRNA and mRNA.
    • Experienced in bioconjugations such as Antibody Drug Conjugates (ADCs) and their corresponding Small Ligand-Drug Conjugates (SLDC), nanoparticle surface functionalization.
    • 10+ years of hands-on experience in synthesis purification and characterization of bioactive macro/molecules, biopolymers, PEGylation, solid phase and solution phase peptide synthesis.
    • Expert in biochemical assays such as the development of cytotoxicity assay, cell surface protein expression analysis using flow cytometry, ELISA, western blot, PCR, and microscopy (TEM, SEM, Fluorescence and Confocal)
    • Proficient in analytical techniques such as NMR (1D and 2D), Chromatography (HPLC, GPC), Mass spectrometry (MALDI-TOF and LC-MS), spectroscopy.
    • Strong interdisciplinary research background in vaccines and cancer chemotherapies.
    • Skilled at conducting due diligence for pharmaceutical technologies.
    • Very good understanding and expertise on Bio-distribution as well as pharmacokinetic analysis.
    • Led several collaborative research projects and demonstrating strong interpersonal and presentation skills.

Publications

  • Biomaterials for mRNA delivery
  • Determination of peptide oligomeric states from thermal unfolding by CD-spectroscopy
  • Membrane interactions of fusogenic coiled-coil peptides: implications for lipopeptide mediated vesicle fusion
  • In vitro and In vivo supramolecular biomembrane engineering using a lipidated coiled-coil motif
  • Coiled-coil peptide motifs as thermoresponsive valves for mesoporous silica nanoparticles
  • Peptide amphiphile nanoparticles enhance the immune response against a CpG adjuvanted influenza antigen
  • Coiled-coil peptide motifs as thermoresponsive valves for mesoporous silica nanoparticles
  • Controlling the rate of coiled coil driven membrane fusion
  • Immobilization of liposomes and vesicles on patterned surfaces by a peptide coiled-coil binding motif
  • Polymer-induced liquid precursor (PILP) phases of calcium carbonate formed in the presence of synthetic acidic polypeptides-relevance to bio-mineralization
  • Influence of pegylation on peptide-mediated liposome fusion
  • Polymersomes enhance the immunogenicity of influenza subunit vaccine
  • In situ modification of plain liposomes with lipidated coiled coil forming peptides Induced membrane fusion through
  • Random co-Poly(Amino Acid)s as Protein-Mimicking Control Agents in CaCO3 Mineralization

Projects

  • Development of conformationally preferred peptide immunogens as candidate vaccine against HIV-infection
  • LEACHATE TREATMENT USING BIOLOGICAL METHODS
  • Design and synthesis of novel Cell Penetrating Peptides (CPPs)
  • Synthetic efforts towards the preparation of 2,3-diallyl indole

Patent

Patent title: Delivery methods

Patent date Issued Oct 26, 2012  Patent issuer and numbereu PCT/EP2012/071320 and WO2014063757

Patent description:

The invention provides a molecule comprising a peptide capable of forming a coiled coil with a complementary peptide, and a steroid moiety. The invention also provides a method of delivering a active agent to a cell, the method comprising contacting the cell with a vehicle comprising a peptide capable of forming a coiled coil with a complementary lipidated peptide and the active agent, wherein the lipid component of the complementary lipidated peptide is integrated into the cell membrane of the cell